Development of a flow cytometry method for detection of bovine multi-cytokines / 生物工程学报

This study aims to develop a method to detect bovine multi-cytokines based on flow cytometry. Previously we have prepared and screened monoclonal antibodies against bovine cytokines IFN-γ, IL-2, TNF-α, IP-10 and MCP-1. These bovine cytokine monoclonal antibodies were fluorescently labeled, and the combination of antibody and cell surface molecules were used to develop the method for detecting bovine multi-cytokines. Subsequently, the developed method was used to determine the cytokine expression profile of Mycobacterium bovis BCG infected bovine peripheral blood mononuclear cells in vitro, and evaluate the cytokine expression level of peripheral blood CD4+ T cells of tuberculosis-positive cattle. The bovine multi-cytokine flow cytometry detection method can effectively determine the cytokine expression of BCG-infected bovine peripheral blood T lymphocytes. Among them, the expression levels of IFN-γ, IL-2, and TNF-α continue to increase after 40 hours of infection, while the expression levels of IP-10 and MCP-1 decreased. The combined detection of IFN-γ, IL-2, and TNF-α on CD4+ T lymphocytes in peripheral blood of cattle can effectively distinguish tuberculosis-positive and tuberculosis-negative samples. This method may facilitate evaluating the level of cellular immune response after bovine pathogen infection and vaccine injection.

Preparation of chitosan hydrochloride stabilized emulsion and its immunostimulatory effect / 生物工程学报

In order to increase the ability of oil-emulsion adjuvant to stimulate cellular immunity, chitosan hydrochloride with positive charge was selected to stabilize oil-in-water emulsion (CHE). In this paper, model antigen ovalbumin was selected to prepare vaccines with emulsion adjuvant, commercial adjuvant or no adjuvant. The emulsion was characterized by measuring the particle size, electric potential and antigen adsorption rate. BALB/c mice were immunized by intramuscular injection. Serum antibody levels, the numbers of IL-4-secreting cells in splenocytes, cytotoxic T lymphocyte (CTL) response, and the expression of central memory T cells were measured to evaluate the immunostimulatory effect. The results showed that chitosan hydrochloride can effectively stabilize the emulsion. The emulsion size is about 600 nm, and the antigen adsorption rate is more than 90%. After immunization, CHE could increase serum antibodies levels and increase IL-4 secretion. Expression of CTL surface activation molecules was also increased to stimulate CTL response further and to increase the CD44+CD62L+ in T cells proportion. CHE as adjuvant can stimulate humoral and cellular immunity more efficiently, and is expected to extend the duration of protection.

Preparation and immunogenicity evaluation of mRNA vaccine against porcine epidemic diarrhea / 生物工程学报

Porcine epidemic diarrhea (PED) is a highly contagious disease that causes high mortality in suckling piglets. Although several licensed inactivated and live attenuated vaccines were widely used, the infection rate remains high due to unsatisfactory protective efficacy. In this study, mRNA vaccine candidates against PED were prepared, and their immunogenicity was evaluated in mice and pregnant sows. The mRNA PED vaccine based on heterodimer of viral receptor binding region (RBD) showed good immunogenicity. It elicited robust humoral and cellular immune responses in mice, and the neutralizing antibody titer reached 1:300 after a single vaccination. Furthermore, it induced neutralizing antibody level similar to that of the inactivated vaccine in pregnant sows. This study developed a new design of PED vaccine based on the mRNA-RBD strategy and demonstrated the potential for clinical application.

Comparative efficacy of different Carbopol concentrations as a stabilizer of live attenuated sheep pox virus vaccine against lumpy skin disease

In Egypt, the lyophilized live attenuated sheep pox virus vaccine has been used for the vaccination of cattle against lumpy skin disease virus to control its economic impact on livestock industry. In this endeavor, we validate the efficacy of Carbopol® as a stabilizer and adjuvant to enhance immunogenicity of such a heterologous sheep pox virus vaccine against lumpy skin disease. Lyophilization of sheep pox virus vaccine stabilized with Carbopol® produced better physical and antigenic properties than freeze-drying with lactalbumin/sucrose stabilizer; this was manifested by superior disc uniformity, thermo-stability at 37oC, and less reduction in virus titer. Immunization of calves' groups with variable sheep pox vaccine doses containing different Carbopol® concentrations revealed that 103.5 TCID50 of sheep pox virus vaccine enclosing 0.5 percent Carbopol® is the field dose of choice. Moreover, it induced protective serum neutralizing index of 2.5 and a ELISA S/P ratio of 36, by the 4th week post vaccination. Besides, the inclusion of 0.5 percent Carbopol® in formulation of the sheep pox virus vaccine was safe in bovines and enhanced cellular immune response to lumpy skin disease virus, as evidenced by increased T cell proliferation. Hence, it is recommended to use Carbopol® as 0.5 percent in preparation of live attenuated sheep pox virus vaccine to confer better protection against lumpy skin disease virus infection(AU)

En Egipto, la vacuna atenuada liofilizada contra el virus de la viruela ovina ha sido utilizado para la vacunación del ganado, contra el virus de la dermatosis nodular contagiosa, para controlar su impacto económico en la industria ganadera. En este trabajo, validamos la eficacia del Carbopol®, como estabilizador y adyuvante, para mejorar la inmunogenicidad de dicha vacuna heteróloga contra la dermatosis nodular contagiosa. La liofilización de la vacuna contra el virus de la viruela ovina estabilizada con Carbopol®, resultó en mejores propiedades físicas y antigénicas que la liofilización con el estabilizador de lactoalbúmina/sacarosa; lo anterior se manifestó en la uniformidad superior del disco, la termoestabilidad a 37°C y la menor reducción del título del virus. La inmunización de grupos de terneros con dosis variables de vacuna contra el virus de la viruela ovina, que contenían diferentes concentraciones de Carbopol®, reveló que la dosis de campo de elección fue 103,5 TCID50 de la vacuna contra el virus de la viruela ovina conteniendo 0,5 por ciento de Carbopol®, la que indujo un índice de neutralización sérica protectora de 2,5 y una relación S/P de ELISA de 36 a la cuarta semana después de la vacunación. Además, la inclusión de Carbopol® al 0,5 por ciento en la formulación de la vacuna contra el virus de la viruela ovina fue segura en los bovinos y potenció la respuesta inmunitaria celular contra el virus de la dermatosis nodular contagiosa, como lo demuestra el aumento de la proliferación de células T. Por lo tanto, se recomienda el uso de Carbopol® al 0,5 por ciento en la preparación de la vacuna viva atenuada contra el virus de la viruela ovina para conferir una mejor protección contra la infección por el virus de la dermatosis nodular contagiosa(AU)

Expression of serum LAIR2 in patients with non-small cell lung cancer and its relationship with cellular immune function / 中华内分泌外科杂志

Objective:To investigate the relationship between serum leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) expression and cellular immune function, prognosis in patients with non-small cell lung cancer.Methods:From April 2016 to April 2017, 90 patients with non-small cell lung cancer who were treated in Shangqiu first people’s Hospital were taken as the lung cancer group, and they were grouped into the survival group and the death group according to whether the patients died within 5 years. Another 84 patients with benign pulmonary mass were selected as the control group. The levels of CD4+[ (28.26±5.14) % vs (47.02±6.73) %], CD8+ [ (23.76±5.84) % vs (30.12±6.03) %] and CD4+/CD8+ [ (1.17±0.30) % vs (1.56±0.50) %] in peripheral blood immune cells were detected by flow cytometry; the serum LAIR2 level was detected by enzyme-linked immunosorbent assay (ELISA) , and the average serum LAIR2 level of patients with non-small cell lung cancer was applied as the boundary, and they were grouped into a low LAIR2 expression group and a LAIR2 high expression group; Pearson correlation was applied to analyze the correlation between serum LAIR2 level and immune cell level in patients with non-small cell lung cancer; Kaplan-Meier curve was applied to analyze the relationship between serum LAIR2 level and 5-year survival rate of patients with non-small cell lung cancer; and multivariate COX regression analysis was applied to analyze the factors affecting 5-year mortality in patients with non-small cell lung cancer.Results:The level of serum LAIR2 [ (69.55±13.12) ng/ml vs. (20.64±7.13) ng/ml] in the lung cancer group was significantly higher than that in the control group, and the levels of CD4 +, CD8 + and CD4+/CD8+ were significantly lower than those in the control group ( P<0.05) . The serum LAIR2 level in patients with TNM stage III+IV (77.32±13.09) ng/ml, poorly differentiated tissue (78.14±13.26) ng/ml, and lymph node metastasis (79.02±13.81) ng/ml was significantly higher than that in patients with TNM stage I+II (64.37±12.89) ng/ml, medium/well differentiated tissue (64.32±12.73) ng/ml, and no lymph node metastasis (62.92±12.85) ng/ml ( P<0.05) . The levels of CD4 +, CD8 + and CD4 +/CD8 + in the LAIR2 high expression group were significantly lower than those in the LAIR2 low expression group ( P<0.05) . Serum LAIR2 level in patients with non-small cell lung cancer was negatively correlated with CD4+, CD8+ and CD4+/CD8+ levels ( r=-0.510, -0.496, -0.494, P<0.05) . The 5-year survival rate of patients with high LAIR2 expression was lower than that of patients with low LAIR2 expression ( r=6.375, P<0.05) . LAIR2 was an independent risk factor for 5-year death in patients with non-small cell lung cancer ( P<0.05) . Conclusion:LAIR2 is highly expressed in serum of patients with non-small cell lung cancer, and its expression level is closely related to cellular immune function and prognosis.

Analysis of the SARS-CoV-2 specific antibodies and immune function of COVID-19 patients one year after discharge / 中国胸心血管外科临床杂志

@#Objective    To summarize the results of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG antibody, total antibody and cellular immune function of COVID-19 convalescent patients one year after discharge, and to analyze the correlation between the SARS-CoV-2 antibody and the indexes of immune function. Methods    A total of 41 confirmed COVID-19 patients discharged from Chengdu Public Health Clinical Medical Center from January to April 2020 and followed up one year after discharge were included in the study as the research group, including 18 males and 23 females with an average age of 47.83±12.95 years. The results of SARS-CoV-2 IgG, total antibody and immune function indexes one year after discharge were collected in order to discuss the correlation of SARS-CoV-2 and cellular immune function. A total of 40 healthy employees of the hospital vaccinated against COVID-19 were randomly selected as the vaccine group, including 10 males and 30 females with an average age of 43.90±6.86 years. The SARS-CoV-2 antibodies between the two groups were compared. Results    CD8+T cell count was higher and CD4+T/CD8+T was lower in male patients than those in female patients (all P<0.05). The IgG and total antibodies in patients with re-detectable positive RNA test were both higher than those in patients without re-detectable positive RNA test, but the differences were not statistically significant (P=0.158, 0.060). The positive rate of SARS-CoV-2 IgG in the research group was 80.5% (33/41). SARS-CoV-2 IgG was positively correlated with total antibody (P<0.001). There was a positive correlation between CD4+T cell count and SARS-CoV-2 IgG (r=0.455, P=0.003). The positive rate of SARS-CoV-2 IgG, SARS-CoV-2 IgG amount and total antibody amount in the research group were significantly higher than those in the vaccine group (all P<0.001). Conclusion    SARS-CoV-2 IgG of most COVID-19 patients one year after discharge is positive, and their SARS-CoV-2 total antibody is significantly higher than people vaccinated against COVID-19, which suggests that patients infected with SARS-CoV-2 can obtain lasting protection, but the protection may be gradually weaken over time. The degree of antibody attenuation in patients with re-detectable positive RNA test may be weaker. In the convalescence stage, the dynamics of SARS-CoV-2 IgG may be closely related to cellular immune function.

In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L) infantum chagasi infection

Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-γ, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-γ. Conclusion Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL.(AU)

Effects of Shenyankangfu tablets combined with losartan on cellular immune function, inflammatory factors and renal function in patients with chronic glomerulonephritis / 中国基层医药

Objective@#To investigate the effects of Shenyankangfu tablets combined with losartan on cellular immune function, inflammatory factors and renal function in patients with chronic glomerulonephritis.@*Methods@#From March 2017 to March 2018, 94 patients with chronic glomerulonephritis admitted to Hangzhou Dajiangdong Hospital were randomly divided into observation group (47 cases) and control group (47 cases). The control group was treated with losartan, and the observation group was treated with Shenyankangfu tablets on the basis of the control group.The two groups were treated for 12 weeks.The changes of cellular immune function, inflammatory factors and renal function were compared between the two groups before and after treatment.@*Results@#After treatment, the levels of CD4＋(71.98±3.28)%, CD4＋(40.01±3.86)% and CD4＋/CD8＋ (1.54±0.23) in the observation group were higher than those in the control group [(64.32±4.01)%, (34.87±1.96)% and (1.23±0.16)] (t=10.137, 8.140, 7.585, all P<0.05). After treatment, the level of IL-2 in the observation group [(3.18±0.59)μg/L] was higher than that in the control group [(2.37±0.71)μg/L], while the levels of IL-6[(227.42±12.41)ng/L] and TNF-α [(2.45±0.29)ng/L] in the observation group were lower than those in the control group [(289.64±19.36)ng/L and (3.38±0.65) ng/L] (t=6.015, 18.549, 8.958, all P<0.05). After treatment, the levels of Scr [(123.49±8.97)μmol/L], Upro[(0.76±0.18)g/24h] and BUN[(6.71±0.5)mmol/L] in the observation group were lower than those in the control group [(159.51±7.41)μmol/L, (1.28±0.23)g/24h, (7.91±0.47)mmol/L](t=21.224, 12.206, 11.136, all P<0.05). The total effective rate of the observation group (93.62%) was higher than that of the control group (72.34%) (P<0.05).@*Conclusion@#Shenyankangfu tablets combined with losartan can improve the cellular immune function and renal function in patients with chronic glomerulonephritis, and can reduce inflammatory reaction.The curative effect is remarkable, which is worthy of clinical reference.

Analysis of the characteristics of cellular immune function and its prognostic risk factors in patients with community-acquired pneumonia / 中华危重病急救医学

Objective To analyze the characteristics of cellular immune function and its prognostic risk factors in patients with community-acquired pneumonia (CAP). Methods The clinical data of patients diagnosed as CAP admitted to department of respiratory and critical care medicine of the Second Affiliated Hospital of Anhui Medical University from June 2018 to February 2019 were retrospectively analyzed. The patients were divided into survival group and death group according to the mortality at discharge and 28-day survival after hospital discharge; in addition, they were divided into bacterial group, fungi group and mixed infection group according to pathogen results at discharge. The differences of general clinical characteristics, arterial blood gas analysis indexes, plasma albumin, cellular immune function, inflammatory cytokines, the length of hospital stay among groups were analyzed. The correlation between the prognosis-related indicators in patients were analyzed by Pearson test or Spearman test, and Logistic regression model was used to analyze the risk factors of patients in non-survival group. Results 106 patients were finally enrolled, 69 of whom were survived, and 37 dead. Among 56 patients with pathogen results, 27 were diagnosed as bacterial infection, 11 as fungal infection, and 18 with mixed infection. Compared with the survival group, plasma albumin level, total T cell count, CD4+ T cell count, CD8+ T cell count were decreased in the death group, temperature, pH, acute physiology and chronic health evaluationⅡ(APACHEⅡ), procalcitonin (PCT), interleukin-6 (IL-6), C-reactive protein (CRP) were increased, and the length of hospital stay was significantly shortened. While there was no significant difference in gender, age, mean arterial pressure, arterial oxygen pressure, CD4+/CD8+, regulation T cell/effect T cell (TREG/TEF) between the two groups. Correlation analysis showed that plasma albumin, the length of hospital stay, APACHEⅡ score, CD4+ T cell count, CD8+ T cell count, CD4+/CD8+, PCT, IL-6, CRP were correlated with death (r value was -0.480, -0.209, 0.203, -0.279, -0.270, 0.271, 0.247, 0.410, 0.329, all P <0.05). Logistic regression analysis showed that plasma albumin, CD4+ T cell count, CD8+ T cell count, CD4+/CD8+decreased; APACHEⅡ score, PCT, IL-6, and CRP increased; the length of hospital stay were correlated with death, and all were independent risk factors for death in CAP patients (all P < 0.05). Correlation analysis showed that the APACHEⅡ score was negatively related to plasma albumin (r = -0.375, P < 0.05), positively related to CRP and IL-6 (r value was 0.363 and 0.333 respectively, both P < 0.05); negative correlation between plasma albumin and IL-6 (r = -0.372, P < 0.05), PCT and CD4+ T cell count (r = -0.354, P < 0.05), CRP and the length of hospital stay (r = -0.356, P < 0.05). There were no significant correlations between the others. There was no significant difference in cellular immune function or inflammatory factor expression between different pathogenic infections. Conclusions Cellular immune dysfunction, hypoproteinemia, APACHEⅡ score and elevated inflammatory index are all influential factors for the death of CAP patients. Apart from conventional anti-infective treatment, the symptoms of hypoproteinemia and cellular immune function can predict the severity and prognosis of CAP patients.

Effects of Shenyankangfu tablets combined with losartan on cellular immune function,inflammatory factors and renal function in patients with chronic glomerulonephritis / 中国基层医药

Objective To investigate the effects of Shenyankangfu tablets combined with losartan on cellular immune function,inflammatory factors and renal function in patients with chronic glomerulonephritis.Methods From March 2017 to March 2018,94 patients with chronic glomerulonephritis admitted to Hangzhou Dajiangdong Hospital were randomly divided into observation group (47 cases) and control group (47 cases).The control group was treated with losartan,and the observation group was treated with Shenyankangfu tablets on the basis of the control group .The two groups were treated for 12 weeks.The changes of cellular immune function ,inflammatory factors and renal function were compared between the two groups before and after treatment.Results After treatment, the levels of CD +4 (71.98 ±3.28)%,CD+4 (40.01 ±3.86)%and CD +4 ／CD+8 (1.54 ±0.23) in the observation group were higher than those in the control group [(64.32 ±4.01)%,(34.87 ±1.96)% and (1.23 ±0.16)] ( t ＝10.137,8.140, 7.585,all P＜0.05).After treatment,the level of IL－2 in the observation group [(3.18 ±0.59)μg／L] was higher than that in the control group [(2.37 ±0.71) μg／L],while the levels of IL －6 [(227.42 ±12.41) ng／L] and TNF－α[(2.45 ±0.29) ng／L] in the observation group were lower than those in the control group [(289.64 ± 19.36)ng／L and (3.38 ±0.65) ng／L] (t＝6.015,18.549,8.958,all P＜0.05).After treatment,the levels of Scr [(123.49 ±8.97) μmol／L],Upro[(0.76 ±0.18) g／24h] and BUN[(6.71 ±0.5) mmol／L] in the observation group were lower than those in the control group [(159.51 ±7.41) μmol／L, ( 1.28 ±0.23 ) g／24h, (7.91 ± 0.47)mmol／L]( t ＝21.224,12.206,11.136, all P ＜0.05).The total effective rate of the observation group (93.62%) was higher than that of the control group (72.34%) (P＜0.05).Conclusion Shenyankangfu tablets combined with losartan can improve the cellular immune function and renal function in patients with chronic glomerulone－phritis,and can reduce inflammatory reaction.The curative effect is remarkable ,which is worthy of clinical reference.

Comparison of immunoadjuvant activities of four bursal peptides combined with H9N2 avian influenza virus vaccine

The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.

Immune response elicited by graphene oxide-based nanovaccine / 国际生物医学工程杂志

Objective To study the antigen-specific immune response induced by the graphene oxide (GO) in mice.Methods OVA-loaded GO nano-immunocomplexes (GO-OVA) were prepared by co-incubation of nano GO with model antigen ovalbumin (OVA).Nano GO was characterized by atomic force microscopy and laser particle sizeanalyzer.The cytotoxicity of GO to mouse bone marrow dendritic cells (BMDCs) was detected by cell counting kit (CCK-8).The GO-OVA uptake of BMDCs were observed by fluorescent staining.C57BL/6 mice were divided into OVA group,aluminum adjuvant OVA (Al-OVA) group and GO-OVA group (6 mice in each group) by body weight for in vivo immunization.The levels of OVA-specific antibody IgG (total IgG,IgG1,and IgG2a) in serum of mice were detected by enzyme-linked immunosorbent assay (ELISA).The T lymphocyte subsets in spleen and inguinal lymph nodes of mice were detected by flow cytometry.Results The average particle size of the prepared nano GO was (294.34±4.68) nm,and the polydispersity coefficient was 0.208.Nano GO has less toxicity to mouse BMDCs.The results of in vitro experiments indicated that GO-OVA nanovaccine can be efficiently internalized by mouse BMDCs.The antigen-specific IgG antibodies induced by the GO-OVA was similar to that of aluminum adjuvant and the difference was not statistically significant (P＞0.05),and the Th1-type response was predominant.The proportions of CD4+ and CD8+ T lymphocytes in the spleen and inguinal lymph nodes in GO-OVA group were significantly higher than those in OVA and Al-OVA groups,and the differences were statistically significant (all P＜0.05).Conclusions GO-OVA nano-immunocomplexes can induce both humoral and cellular immune responses in mice,which provides basis for the development of novel vaccine vectors and adjuvants.

Effect of Feiliuping ointment combined with chemotherapy on cellular immune function and related inflammatory factors in patients with lung cancer / 中草药

Objective To study the effect of Feiliuping ointment combined with chemotherapy on the cellular immune function and related inflammatory factors in patients with lung cancer. Methods A total of 202 patients with non-small cell lung cancer (NSCLC) admitted to Henan Cancer Hospital from May 2014 to December 2016 were selected as subjects. They were randomly divided into the simple chemotherapy group (control group) and Feiliuping Ointment combined with chemotherapy group (observation group), and there were 101 cases in each group. The short-term curative effect, cellular immune function, and levels of inflammatory factors before and after treatment were observed and the adverse reactions were recorded. Results The effective rate and stability rate in the observation group were significantly higher than those in the control group (P < 0.05). Levels of CD3+, CD4+, CD4+/CD8+, and NK in the observation group after treatment were significantly higher than those in the control group (P < 0.05), while CD8+ was significantly lower than the control group (P < 0.05). Compared with the control group, the levels of serum IL-6, TNF-α, and hs-CPR were significantly lower in the observation group after treatment (P < 0.05). The incidence of adverse reactions in the observation group after treatment was significantly lower than that in the control group (P < 0.05). Conclusion Feiliuping Ointment combined with chemotherapy can effectively improve the clinical curative effect on lung cancer, regulate the immune mechanism, reduce the levels of inflammatory factors, improve the condition, and reduce the occurrence of adverse reactions in patients, thus deserving promoting.

Effect of 3D laparoscopic hepatectomy in glissonian approach for hepatocellular carcinoma on the cellular immune function / 实用医学杂志

Objective To compare the effects of 3D laparoscopic hepatectomy (3D-LH) and open hepatectomy(OH)in glissonian access for hepatocellular carcinoma(HCC)on postoperative immunosuppression by analyzing cellular immune function. Methods During January 2014 to December 2017,sixty patients randomly scheduled to undergo hepatectomy were divided into 3D-LH group and OH group,with 30 patients in each group. All patients' peripheral blood was sampled to measure cell-mediated-immunologic markers(CD4+,CD8+T cell) before operation and in the mornings of the 1st,7thday after surgery. The effects of different operative methods on cellular immune function in patients organism were investigated. Results The decreased level in 3D-LH group was less than that in OH group in postoperative 1stday. The improvement in the 3D-LH group was more faster than that in the OH group on postoperative 7thday. There was statistical significance between the two groups(P<0.05), respectively. Conclusion When compared to open hepatectomy ,3D laparoscopy liver resection in glissonian approach for HCC,less effects in cellular immune function.

The effect of structured lipid emulsion on cellular immune function in patients with gastrointesti-nal cancer after operation / 临床外科杂志

Objective To investigate the effect of structured lipid emulsion on cellular immune function in patients with gastrointestinal cancer after operation. Methods 76 cases of gastrointestinal cancer patients were chosen as the research object.The patients were divided into the control group and the experimental group according to the nutrition support after the operation,38 cases in each group.The experimental group used 20% structural lipid emulsion injection(medium chain fat milk accounted for 36%,long chain fat milk accounted for 64%,250 ml/ bottles),the control group used 20% physically mixed medium / long chain fat milk(medium chain fat milk and long chain fat milk accounted for 50%, respectively,250 ml/ bottles),and then compared the immune function index of the two groups.Results Immune function index of CD3 +at preoperative,third days and sixth days after the operation in the experi-mental group were 65.92 ± 4.71,60.62 ± 6.33 and 66.51 ± 3.58,respetively.While in the control group were 65.77 ± 4.63,57.86 ± 6.11 and 60.04 ± 3.21.Immune function index of CD4 +at preoperative, third days and sixth days after the operation in the experimental group were 43.75 ± 6.37,38.44 ± 5.68 and 47.98 ± 3.22,respectively.While in the control group were 43.26 ± 6.18,35.32 ± 6.78 and 37.93 ± 7.15,respectively.Immune function index of CD8 +at preoperative,third days and sixth days after the operation in the experimental group were 23.85 ± 7.16,21.96 ± 7.45 and 23.67 ± 5.47,respectively. While in the control group were 23.36 ± 6.69,21.43 ± 7.02 and 22.54 ± 6.12,respectively.Immune function index of CD4 +/CD8 +at preoperative,third days and sixth days after the operation in the experi-mental group were 1.86 ± 0.35,1.72 ± 0.39 and 1.99 ± 0.56,respectively.While in the control group were 1.83 ± 0.31,1.66 ± 0.32 and 1.68 ± 0.41,respectively.Immune function index(CD3 +,CD4 +and CD4 +/CD8 +)in the experimental group were significantly higher than those in the control group after sixth days(P< 0.05).Conclusion Postoperative early structured lipid emulsion nutrition support can correct the immune suppression in patients with gastric intestinal cancer,and improve cellular immune function.

Biocompatibilidade da resina acrílica de prótese ocular: análise in vitro e in vivo / Biocompatibility of ocular prosthesis acrylic resin: in vitro and in vivo analysis

O objetivo desse estudo foi avaliar a influência de diferentes ciclos e métodos de polimerização da resina acrílica (RA) branca de próteses oculares sobre a biocompatibilidade de células da conjuntiva humana e resposta inflamatória do tecido subcutâneo de ratos. Para isso, foram confeccionados corpos de prova em RA termopolimerizados em água aquecida (RNAA), por energia de microondas (RNTM) e quimicamente ativados (RNQA). Para a análise in vivo, a resposta inflamatória desses 3 grupos (n=20/grupo) foi avaliada no tecido subcutâneo de 20 ratos Wistar por 7, 15, 30 e 60 dias (d). Células inflamatórias foram contadas no tecido adjacente ao corpo de prova após coloração com hematoxilina e eosina. A análise imunohistoquímica foi realizada para a detecção de IL-1ß, IL-6, TNFα, IL-17 e CCL20. Para a análise in vitro, diferentes ciclos de polimerização para cada método citado foram avaliados, totalizando 11 grupos (n=8/grupo). Foram realizadas análises de grau de conversão (GC), MTT, Alamar Blue, ELISA, RT-PCR em tempo real e dupla marcação de Anexina V e iodeto de propídio. Dados quantitativos foram submetidos à Análise de Variância e ao teste de Tukey com significância de 5%. Os resultados qualitativos foram comparados visualmente. Na análise in vivo, houve infiltrado inflamatório moderado para os grupos RNTM e RNQA e leve para o grupo RNAA após 7 d. O infiltrado inflamatório e a imunomarcação dos alvos testados diminuiu gradativamente ao longo dos 60 d. O grupo RNTM exibiu mais células inflamatórias, com exceção do grupo RNAA, que apresentou mais eosinófilos e linfócitos após 15 d, e do grupo RNQA, onde foi observado mais macrófagos em 15 d e neutrófilos em 60 d. Os grupos RNAA e RNQA apresentaram maior imunomarcação de IL-1ß após 7 d. O grupo RNQA apresentou maior imunomarcação de IL-1ß (15 e 30 d), IL-6 (30 e 60 d), IL-17 (15 e 30 d) e TNF-α (7 d). Os grupos RNAA e RNTM apresentaram maior imunomarcação de TNF-α nos períodos de 15 e 30 d, enquanto o grupo RNTM, aos 60 d. Na análise in vitro, todos os grupos apresentaram proliferação celular maior que 75%. O ciclo longo de polimerização em microondas apresentou menor GC e percentual de proliferação celular no MTT e resultou em grande liberação de IL-2. No ensaio de Alamar Blue, esse grupo apresentou baixo percentual de proliferação celular, assim como o grupo que recebeu ciclo longo de polimerização em água aquecida e grupos submetidos à ativação química. Maior liberação de IL-6 foi observada nos grupos submetidos à ativação química e de IL-23 para o ciclo curto de polimerização em microondas. Maior expressão gênica de TGF ß1 ocorreu para o grupo que recebeu ciclo longo de polimerização em água aquecida seguido de 30 min de armazenamento em água. Maior expressão gênica de CASP9 ocorreu para o grupo ativado quimicamente sobre a bancada. Pode-se concluir que os métodos de polimerização por meio de energia de microondas (ciclos longo e curto) e por ativação química desencadearam uma resposta inflamatória mais intensa. Dentre os métodos de polimerização recomendados pelo fabricante, a polimerização em água aquecida apresentou resultados mais satisfatórios(AU)

The aim of this study was to evaluate the influence of different cycles and methods of white color acrylic resin (AR) for ocular prosthesis on the biocompatibility of human conjunctival cells and on the inflammatory response of rat subcutaneous tissue. For this, AR specimens were prepared in water bath (NRWB), by microwave energy (NRME), and chemically activated (ANR). For in vivo analysis, the inflammatory response of these 3 groups (n=20/group) was assessed in subcutaneous tissue of 20 Wistar rats at 7, 15, 30 and 60 days (d). Inflammatory cells were counted in the tissue adjacent to specimen after staining with hematoxylin and eosin. The immunohistochemical analysis was performed for the detection of IL-1ß, IL-6, TNFα, IL-17, and CCL20. For in vitro analysis, different cycles of polymerization for each method were evaluated, with a total of 11 groups (n=8/group). The degree of conversion (DC), MTT, ELISA, real-time RT-PCR and Annexin V and propidium iodide assays were performed. Quantitative data were submitted to Analysis of Variance and Tukey test with a 5% significance. Qualitative data were submitted to visual comparison. In in vivo analysis, there was a moderate inflammatory infiltrate for groups NRME and ANR, and a light infiltrate for the group NRWB after 7 d. The inflammatory infiltrate and the immunolabeling of tested targets decreased gradually during the 60 d. The group NRME exhibited the highest number of inflammatory cells, except for the group NRWB, which presented a higher number of eosinophils and lymphocytes after 15 d, and for the group ANR, where a higher number of macrophages and neutrophils were observed at 15 d and at 60 d, respectively. Groups NRWB and ANR showed higher IL-1ß immunolabeling after 7 d. The group ANR had the highest immunolabeling of IL-1ß (15 and 30 d), IL-6 (30 and 60 d), IL-17 (15 and 30 d), and TNF-α (7 d). Groups NRWB and NRME showed greater immunolabeling in the periods of 15 and 30 d, while the group NRME had also high results at 60 d. In in vitro analysis, all groups showed cell proliferation higher than 75%. The long cycle of polymerization using microwave energy resulted in lower DC and lower percentage of cell proliferation in the MTT assay and in large release of IL-2. In the Alamar Blue assay, this group had a low percentage of cell proliferation, as well as the group that received a long cycle of polymerization in water bath and groups submitted to chemical activation. A higher release of IL-6 was observed in groups submitted to chemical activation and of IL-23, for the short cycle of polymerization in microwave. Higher TGF ß1 gene expression occurred for the group that received long cycle of polymerization in water bath followed by 30 min of storage in water. Higher CASP 9 gene expression occurred for the chemically activated group on bench. It can be concluded that the polymerization by microwave energy (long and short cycles) and by chemical activation resulted in higher inflammatory response. Among methods recommended by the manufacturer, the water bath polymerization showed more satisfactory results(AU)

Effects of pegylated interferon on cellular immune function in patients with chronic hepatitis C / 中国生化药物杂志

Objective To investigate the clinical effect of pegylated interferon(IFN)on chronic hepatitis C and its effect on cellular immune function.Methods A total of 92 patients with chronic hepatitis C were enrolled in our hospital from June 2013 to October 2016.They were randomly divided into study group and control group by single and double numbers(admission order),with 46 cases in each group.The control group was treated with recombinant human interferon alpha-2b on the basis of conventional treatment.The study group was treated with pegylated interferon alpha-2a on the basis of conventional treatment.Results After treatment, the rates of RVR, EVR, ETVR and SVR in the study group(52.17%, 71.74%, 76.09% and 54.35%, respectively)were significantly higher than those in the control group(34.78%, 50%, 58.70%, 39.13%, respectively), The difference was statistically significant(P<0.05).There were no significant difference in the cellular immune function index between the two groups before treatment;After treatment,the CD3+, CD4+, CD4+/CD8+ and other immune function indexes of the study group were significantly higher than those before treatment, while the results of CD8+ were significantly lower than before treatment(P<0.05);There was no significant difference in the cellular immune function in the control group compared with before, and the difference of the cellular immune function between the 2 groups after treatment was statistically significant(P<0.05).Conclusion Pegylated interferon(IFN)on the basis of routine treatment can improve the virologic response and cellular immune function in patients with chronic hepatitis C.

The clinical significance of thymopeptides α1 in the treatment of severe sepsis / 中国生化药物杂志

Objective To investigate the application values of thymosin α1 in severe sepsis.Methods Selected 94 patients with severe sepsis in our hospital from September 2014 to October 2016,were randomly divided into observation group(n=45)and control group(n=49),the control group was given routine treatment,the observation group was given thymosin α1 on the basis of conventional treatment,observed two groups before and after treatment T cell subsets and CD14+monocyte human leukocyte antigen(HLA-DR)and so on.Results The observation group the duration of ventilator use and ICU treatment were(12.51±3.82)d and(15.81±3.18)d,significantly shorter than the control group(P< 0.05).There was no significant difference in 28d mortality between the observation group and the control group; After treatment,the CD14+monocytes HLA-DR,CD3+and CD4+T cells in the observation group were significantly improved than before treatment(P<0.05);The HLA-DR,CD3+and CD4+T cells in the observation group after treatment were(36.04± 8.90)％,(58.93±8.74)％and(43.20±9.90)％,significantly higher than those in the control group(P<0.05);The observation group after treatment TNF-α,endotoxin and CRP respectively(56.40±11.78)ng/L,(27.83±9.98)ng/L and(53.20±9.73)g/mL,significantly lower than the control group(P< 0.05).Conclusion Application of thymosin α1 in the treatment of severe sepsis,which can improve the cellular immune function,adjust the state of inflammatory response,shorten the duration of mechanical ventilation and ICU stay.

Effect of Ningdong granule combined with haloperidol on the therapeutic effect and cellular immune function in children with Tourette's syndrome / 中国生化药物杂志

Objective To investigate the clinical effect and changes in cellular immune of children with Tourette 's syndrome in treatment with Ningdong granule and haloperidol.Methods 48 cases of children with Tourette's syndrome from February 2015 to February 2016 in hospital were selected and randomly divided into the observation group and control group with 24 cases in each group.The patients of two groups were all treated with conventional treatment, including mental relaxation, reverse muscle relaxation therapy and self-training.The patients of control group were treated with haloperidol, and on the basis of the control group, the patients of observation group were treated with Ningdong granule.The clinical effect, the levels of TNF-ɑ, IL-12, CD3, CD4, CD8, CD4/CD8 were compared before and after treatment between two groups, and recorded the incidence of adverse reactions in two groups of children.Results After the treatment, the total effective rate of the observation group was 91.6%, which was significantly better than the control group 66.6%, the difference was statistically significant (P<0.05).The levels of TNF-ɑ, IL-12, CD8 of the observation group were significantly lower than those of the control group (P<0.05), the levels of CD3, CD4, CD4/CD8 of the observation group were significantly higher than those of the control group, the difference was statistically significant (P<0.05).There were constipation, dizziness, drowsiness, fatigue, poor appetite, tachycardia and other adverse reactions occurred in both two groups, and the incidence of adverse reactions in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05).Conclusion The combination of Ningdong granule and haloperidol in the treatment of children with Tourette 's syndrome is effective and can improve the cellular immune function .

Static absorption compound anesthesia with intravenous anesthesia alone stress reaction and cellular immune function with gastric cancer surgery / 中国现代普通外科进展

Objective:To explore the analysis of the static absorption compound anesthesia with intravenous anesthesia alone stress reaction and cellular immune function in patients with gastric cancer surgery.Methods:From March 2014 to March 2014 were lines of gastric cancer patients,78 cases of elective surgical procedureS,using the random number table method was divided into control group and experimental group,each group of 39 cases,control group given simple intravenous anesthesia,patients given static absorption compound anesthesia,compared two groups of patients with preoperative 30 min (T0),cut the skin (T1),bi (T2),tube drawing immediate (T3),24 h after surgery (T4) different time points,such as serum C-reactive protein (CRP) and interleukin 2 (IL-2),the level of interleukin 8 (IL-8),CD4,CD8,the number of NK cells,and CD4/CD8 ratio.Re· sults:T2 IL-2,IL-8 point test group dropped to the lowest level,CRP rose to the highest,to T4 point back to the level before anesthesia,IL-2 and the control group,while the level of IL-8 T3 point minimum,CRP rose to the highest,and T4 point is still not fully recovered to the level before anesthesia,CRP T1 and T4 each point test group,IL-2 and IL-8 cytokine levels were significantly better than the control group,with significant difference (P＜0.05).T1 point,two groups of patients with CD4,NK cells and CD4/CD8 ratios were significantly decreased,CD8 levels increased significantly,CD4 T3 point test group began to rebound,NK cells and CD4/CD8 ratio,level of CD8 began to decline,and T4 point before anesthesia recovery level,and T4 point control indicators are still relatively preoperative levels have obvious deviation,T1 and T4 each point group CD4,NK cells and CD4/CD8 ratio than the control group obviously on the higher side,had clear statistical significance(P＜0.05),and each point CD8 levels have no obvious difference between groups(P＞0.05).Conelusions:Different anesthetic methods on gastric cancer surgery the patient's level of stress reaction and cell immune function all can produce certain effect,compared with the conventional simple intravenous anesthesia,the static absorption compound anesthesia can obviously relieve open perioperative stress response in patients with gastric cancer,reduce the inhibition of cellular immune level,is a kind of can effectively guarantee the anesthesia effect and prognosis of patients with ideal anesthesia method.